Next-generation sequencing in study of hepatitis B virus variation
10.3969/j.issn.1001-5256.2015.04.009
- VernacularTitle:新一代测序技术在HBV变异研究中的应用
- Author:
Xinxin ZHANG
;
Mingjie WANG
- Publication Type:Journal Article
- Keywords:
hepatitis B virus;
mutation;
next generation sequencing
- From:
Journal of Clinical Hepatology
2015;31(4):514-519
- CountryChina
- Language:Chinese
-
Abstract:
The high mutation rate during hepatitis B virus (HBV)replication leads to HBV quasispecies.The study of HBV quasispecies has an important significance for the hepatitis B pathogenesis,prognosis,and outcome prediction.Recently,the next-generation sequen-cing (NGS)is extensively used in many biological and medical fields due to its high throughput,ultra-deep coverage,and high sensitivity, which also brings new strategies to HBV quasispecies studies.This article describes the working principles and features of several commonly used NGS technologies,reviews the application of NGS technologies in HBV quasispecies detection in recent years,and particularly discus-ses the variations in different regions of HBV genome and the population characteristics of HBV quasispecies.For now,NGS technologies used in HBV variation detection mainly presents the advantages of high sensitivity and high throughput.However,how to lower the cost,in-crease the accuracy of sequencing,and standardize the procedure of massive sequencing data management and personalized analysis are still challenging problems.The issue about NGS that matters at present and urgently needs to be solved is how to overcome its limitations,and then put it into HBV-related studies and ultimately clinical application.
