The effects of human hepatocarcinoma HepG2 cell culture supernatant on T lymphocyte activity
- VernacularTitle:人肝癌细胞系HepG2培养上清液对人淋巴细胞活性的影响
- Author:
Kechang ZHANG
;
Junying WU
;
Baiqing LI
- Publication Type:Journal Article
- Keywords:
human hepatocarcinoma;
lymphocyte;
activation;
proliferation;
apoptosis
- From:
Journal of Clinical Hepatology
2009;25(3):204-206
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the role of human hepatocarcinoma HepG2 cell culture supernatant on T lymphocyte of the activation, proliferation, IL-2 secretion and inducing apoptosis in vitro. To explore the mechanism of immune escape in tumor.Methods Peripheral blood mononuclear cells (PBMC) were cultured with different concentration of tumor cell culture supernatant. Cell proliferation was examined with standard cytometry and MTT. The apoptotic rate and immune phenotype were analyzed by FACS. The level of IL-2 was determined with ELISA.Results Adding HepG2 supernatant, there was no significant influence in activation of lymphocyte, but the proliferation rat and the secretion of IL-2 were obviously decreased. The lymphocyte apoptosis rate was raised.Conclusion The tumor cell may produce a certain soluble protein, thus escape from the immune surveillance of tumor-bearing host.
