The associat ion of Her-2 amplification and overexpression with clinicopathological faetures in adenocar-cinoma of the gastroesop hageal junction
10.11904/j.issn.1002-3070.2014.05.006
- VernacularTitle:胃食管结合部癌Her－2基因扩增及蛋白表达与临床病理关系
- Author:
Hongbin HAN
;
Baowei HAN
;
Zhaohui LI
;
Shuaijun DONG
;
Shuiafeng LIU
- Publication Type:Journal Article
- Keywords:
Adenocarcinoma of the gastroesophageal junction;
Her-2;
Immunohistochemistry;
Fluores-cence in situ hybridization
- From:
Practical Oncology Journal
2014;(5):410-414
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the protein expression and gene amplification of human epidermal growth factor receptor-2(Her-2)in adenocarcinoma of the gastroesophageal junction and its association with the clinicopathological features .Methods Immunohistochemical method and fluorescence in situ hybridization ( FISH) were used to detect Her -2 expression and gene amplification in 80 surgically resected gastroesophageal junction cancer tissue .We further analyzed the relationship between the gastroesophageal junction cancer histolog -ical differentiation ,as well as depth of invasion ,clinical stage and lymph node metastasis .Results Her-2 am-plification of gastroesophageal junction carcinoma by FISH was 22.5%,while the overexpression by IHC was 15%.Her-2 gene amplification and protein overexpression were correlated with the differentiation ,clinical TNM stage,invasive depth and lymph node metastasis of the carcinoma (all P<0.05).Her-2 expression was not asso-ciated with the patients age,gender,invasive depth and lymph node metastasis(P<0.05).Conclusion Her -2 protein expression and gene amplification may be a prognostic indicator for the patients with gastroesophageal junction cancer .The joint detection of Her -2 protein expression level and the gene amplification can guide the prognosis and therapy for adenocarcinoma of the gastroesophageal junction .
