Time-Dependent FTIR Spectral Changes in Rats of Massive Hemorrhage Death during the Later Postmortem Period
10.3969/j.issn.1004-5619.2012.04.001
- VernacularTitle:Time-Dependent FTIR Spectral Changes in Rats of Massive Hemorrhage Death during the Later Postmortem Period
- Author:
Shiying LI
;
Yu SHAO
;
Zhengdong LI
;
Li LI
;
Yuanyuan CHEN
;
Yijiu CHEN
;
Ping HUANG
- Publication Type:Journal Article
- Keywords:
forensic pathology;
spectroscopy,Fourier transform infrared;
hemorrhage;
postmortem interval;
rats
- From:
Journal of Forensic Medicine
2012;28(4):241-246
- CountryChina
- Language:Chinese
-
Abstract:
The aim of the current study was to investigate the spectra in the different organs of the rats which died of massive hemorrhage; to explore their spectral changes 15 days postmortem and the best mathematical model with different band absorption ratio changes to postmortem interval(PMD; and to compare the spectral changes of different temperature.Thirty male Sprague-Dawley rats were sacrificed by cutting abdominal aorta,and the cadavers were divided equally and kept at 4 ℃,20℃ and 30℃ in the control chamber.From the same rat,seven different organs were sampled at intervals of 1-15 days postmortem,and then measured by Fourier transfom infrared (FTIR)spectrometer.Six mathematical model functions were explored.The absorbance of bands and band absorbance ratios of absorption peak in each organ showed a time-dependent increase or decrease,most band absorbance ratios remaining stable for 7-15 days postmortem.Cubic model functions of the various bands absorbance ratios against PMI showed a stronger related coefficient.The absorbance bands with obvious changes at 20 ℃ showed stabilized tendencies at 4 ℃ and significant changes at 30 ℃ within 15 days postmortem.In addition,FTIR spectroscopy revealed a time-dependent metabolic process,with potential of being used to estimate PMI during 7 days postmortem,which merits further investigation.
