Consistency Study of PowerPlex?21 Kit and GoldeneyeTM 20A Kit and Forensic Application
10.3969/j.issn.1004-5619.2014.03.009
- VernacularTitle:PowerPlex?21与GoldeneyeTM 20A试剂盒的一致性验证及法医学应用
- Author:
He REN
;
Ying LIU
;
Qingxia ZHANG
;
Zhangping JIAO
- Publication Type:Journal Article
- Keywords:
forensic genetics;
polymorphism,genetic;
STR;
D1S1656;
consistency verifying;
Beijing;
Han nationality
- From:
Journal of Forensic Medicine
2014;(3):191-193
- CountryChina
- Language:Chinese
-
Abstract:
To ensure the consistency of genotype results for PowerPlex?21 kit and GoldeneyeTM 20A kit. Methods The STR loci were amplified in DNA samples from 205 unrelated individuals in Beijing Han population. And consistency of 19 overlap STR loci typing were observed. The genetic polymorphism of D1S1656 locus was obtained. Results All 19 overlap loci typing showed consistent. The proportion of peak height of heterozygous loci in two kits showed no statistical difference (P>0.05). The observed heterozygosis of D1S1656 was 0.878. The discrimination power was 0.949. The excluding probability of paternity of triplet was 0.751. The excluding probability of paternity of diploid was 0.506. The polymor-phism information content was 0.810. Conclusion PowerPlex?21 kit and GoldeneyeTM 20A kit present a good consistency. The primer design is reasonable. The polymorphism of D1S1656 is good. The two kits can be used for human genetic analysis, paternity test, and individual identification in forensic practice.
