Determination of Five Pesticides in Fishpond by SPE-GC/MS

VernacularTitle:SPE-GC/MS 法分析鱼塘水中的5种农药
Author: Dawei LIN ; Yan ZHANG ; Honglei SUN ; Wenhai LI ; Kai SHAO
Publication Type:Journal Article
Keywords: forensic toxicology; pesticides; gas chromatography-mass spectrometry; solid phase extraction
From: Journal of Forensic Medicine 2015;(1):41-43
CountryChina
Language:Chinese
Abstract: Objective To establish the solid phase extraction (SPE) with GC/MS technology for fish poi-soning cases to determine five pesticides in fishpond. Methods By three solid phase extraction column including Oasis HLB cartridge, Bond Elut C18 and SampliQ C18, the recovery rate was compared to ex-tract and purify five pesticides in fishpond. The effects of different kinds and dosages of eluents on ex-tract rate were also reviewed. Results Using Bond Elut C18 as solid phase extraction column and 3 mL benzene as eluent, the linear range of mass concentration of five pesticides in fishpond was 1-50 μg/mL, and the correlation coefficient was 0.996 2-0.999 6. The limit of detection was 3.4-26 μg/L and the re-covery was 61.49%-102.48%. The relative standard deviations was less than or equal to 3.01%. Conclu-sion With high sensitivity, good accuracy and precision, SPE -GC/MS has simple and quick operation and less solvent. It can be applied to determination of five pesticides in fishpond.