Correlation between Percentages of PMN, MNC, FBC and Wound Age after Skeletal Muscle Injury in Rats
10.3969/j.issn.1004-5619.2014.03.002
- VernacularTitle:大鼠骨骼肌损伤后PMN、MNC及FBC百分率与损伤时间关系
- Author:
Tianshui YU
;
Dawei GUAN
;
Rui ZHAO
;
Haidong ZHANG
;
Rufeng BAI
- Publication Type:Journal Article
- Keywords:
forensic pathology;
wounds and injuries;
muscle,skeletal;
polymorphonuclear leucocytes;
mononuclear cells;
fibroblastic cells;
rats
- From:
Journal of Forensic Medicine
2014;(3):166-168
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the percentages of polymorphonuclear leukocytes (PMN), mononuclear cells (MNC) and fibroblastic cells (FBC) in different post-traumatic intervals after skeletal muscle me-chanical injury in rats. Methods The rat model of skeletal muscle mechanical injury was established. The rats were divided into injured groups (6 h, 12 h, 1 d, 3 d, 7 d, 10 d and 14 d after injury ) and con-trol group. The percentages of PMN, MNC and FBC in different post-traumatic intervals after skeletal muscle mechanical injury were assessed with HE staining and image analysis. Results At post-injury 6-12 h, the percentages of PMN and MNC infiltration appeared in injured sites and that of PMN reached peak. At 1 d, the percentage of MNC infiltration appeared and reached peak, while that of PMN de-creased. At 3-7 d, the percentage of FBC gradually increased, while that of PMN and MNC decreased. At 10-14 d, the percentage of FBC reached peak. Conclusion The percentages of PMN, MNC and FBC in injured zones showed time-dependent changes, which might be used as reference index for determination of age of skeletal muscle injury.
