Cardiac Ultrastructure and Changes of HSP70 and HIF-1α Expression in Electric Shock Death Rats
10.3969/j.issn.1004-5619.2015.04.002
- VernacularTitle:电击死大鼠心脏超微结构及HSP70、HIF－1α表达变化
- Author:
Guozhong ZHANG
;
Ruili LI
;
Guowei FENG
;
Haitao BI
;
Songjun WANG
;
Bin CONG
;
Min ZUO
- Publication Type:Journal Article
- Keywords:
forensic pathology;
electric injuries;
heart;
heat shock protein 70 (HSP70);
hypoxia inducible factor-1α (HIF-1α);
rats
- From:
Journal of Forensic Medicine
2015;(4):262-265
- CountryChina
- Language:Chinese
-
Abstract:
Objective T o observe cardiac ultrastructure and the expression of heat shock protein 70 (H SP70) and hypoxia inducible factor-1α (H IF-1α) in electric shock death rats and to explore the application of these indexes as the basis of m edical identification in electric shock death. Methods Seventy-tw o SD rats w ere random ly divided into electric shock death group, postm ortem electric shock group and the control group. T he changes of m yocardial ultrastructure w ere observed by transm ission electron m icro-scope, and the expressions of m yocardial H SP70 and H IF-1α w ere observed by im m unohistochem ical technology. Results M yocardial m yofibril fracture, m itochondrial cristae and m em brane dissolution, and disordered arrangem ent of Z lines and M lines w ere observed in electric shock rats. H SP70 and H IF-1αw ere strong positive expressions in the electric shock death group, significantly com pared w ith the con-trol and postm ortem electric shock groups (P<0.05). Conclusion T he expressions of H SP70 and H IF-1αw ere obviously increased in electric shock death group, w hich m ay be used as the diagnostic indicator of electric shock death.
