Relation between Injury Time and the Expression of COX6C mRNA in Skeletal Muscle of Rats after Contusion
10.3969/j.issn.1004-5619.2015.03.001
- VernacularTitle:大鼠肌肉挫伤后COX6C mRNA的表达与损伤时间关系
- Author:
Junhong SUN
;
Lei ZHANG
;
Xiaowei WANG
;
Qiuxiang DU
;
Jian LU
;
Yingyuan WANG
- Publication Type:Journal Article
- Keywords:
forensic pathology;
w ounds and injuries;
muscle;
cytochrome coxidase subunitⅥc;
rats
- From:Journal of Forensic Medicine
2015;(3):177-180
- CountryChina
- Language:Chinese
-
Abstract:
Objective T o investigate the relation betw een injury tim e and the expression of cytochrom e c oxidase subunitⅥc (COX6C) m R N A in skeletal m uscle of rat after contusion. Methods A total of fifty-four SD rats w ere divided into the control group and the contusion groups (0.5,1,6,12,18, 24, 30, and 36 h after contusion), random ly. T he contusion m odel w as established by free fall drop of gravity ham-m er. A t corresponding tim e point after contusion, the regular histology w as exam ined and expression level of COX6C m R N A w as tested by real-tim e PC R after extraction of total R N A from the tissues. Results T he m ain pathological features of 6 h after injury included edem a and hem orrhage in m yocytes w ith no inflam m atory cells found. A fter 6 hours, the findings included m yocyte degeneration and necro-sis, inflam m atory cells infiltration, and fibrous connective tissue proliferation in the contused zone. T he expression level of COX6C m R N A w as higher than that of the control group w ithin 6 h after contusion. T he expression level w as low er than that of the control group from 6-36 h after contusion. Conclusion T he level of COX6C m R N A expresses in a regular w ay after contusion. It m ay be useful for estim ating w ound age in com bination w ith the results of pathological features.
