Diagnostic Significance of BAT in Anaphylaxis to Non-ionic Contrast Media
10.3969/j.issn.1004-5619.2015.03.004
- VernacularTitle:BAT在非离子型造影剂过敏反应中的诊断价值
- Author:
Haoyue ZHANG
;
Sujun XU
;
Xiaoxian TANG
;
Jijun NIU
;
Xiangjie GUO
;
Cairong GAO
- Publication Type:Journal Article
- Keywords:
forensic pathology;
anaphylaxis;
non-ionic contrast m edia;
basophil activation test;
C D 63;
m ast cell carboxypeptidase A 3;
SC 5b-9
- From:
Journal of Forensic Medicine
2015;(3):188-190
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the diagnostic significance of basophil activation test (B A T) in ana-phylaxis to non-ionic contrast media through testing the content of CD 63, m ast cell-carboxypeptidase A 3 (M C-CPA 3), and term inal com plem ent com plex SC5b-9 of the individuals by testing their levels in the norm al im m une group and the anaphylaxis groups to β-lactam drugs and non-ionic contrast media. Methods The CD 63 expression of basophilic granulocyte in blood w as detected by flow cytom etry. The levels of M C-CPA 3 in blood serum and SC5b-9 in blood plasm a w ere detected by ELISA . Results The CD 63 expression of basophilic granulocyte in blood, the levels of M C-CPA 3 and SC5b-9 of anaphylaxis to non-ionic contrast media and β-lactam drugs w ere significantly higher than that in norm al im m une group (P<0.05). Conclusion There is activation of basophilic granulocytes, m ast cells and com plem ent system in anaphylaxis to non-ionic contrast media. B A Tcan be used to diagnose the anaphylaxis to non-ionic contrast media.
