Analysis of GHB and Its Precursors in Urine and Their Forensic Application

VernacularTitle:尿液中γ-羟基丁酸及其前体物质的检测和应用
Author: Yan SHI ; Xiaopei CUI ; Ping XIANG ; Baohua SHEN
Publication Type:Journal Article
Keywords: forensic toxicology; chrom atography,liquid; tandem m ass spectrom etry; gam m a-hydroxybu-tyric acid; urine
From:Journal of Forensic Medicine 2015;(3):200-203
CountryChina
Language:Chinese
Abstract: Objective To establish the m ethod to analyze γ-hydroxybutyric acid (GHB ) and its precursors 1,4-butanediol (1,4-B D ) and gam m a-butyrolactone (GBL) in urine through LC-M S/M S and provide evi-dence for related cases. Methods GHB-d6 and M O R-d3 were used as the internal standard. The urine sam ple w as separated by LC after protein precipitation w ith m ethanol. The electrospray ion source w as for ionization. E ach com pound w as detected through m ultiple-reaction m onitoring (M R M ) m ode. Results The lim its of detection of GHB and its precursors 1,4-B D and GBLwere 0.1, 0.1 and 2μg/m L. The accuracy w as 87.6% -98.1% . The intra-day and inter-day precisions were less than 15% and m atrix ef-fects were higher than 80% . Conclusion The m ethod is high sensitive, sim ple, rapid, specific and w ith high reliability. This study has provided technical support and basic data for forensic cases involving GHB .