Relationship between Injury Time and Expressions of EPO and Its Receptors in Rats Brain after Cerebral Injury
10.3969/j.issn.1004-5619.2016.02.003
- VernacularTitle:大鼠脑损伤后EPO及其受体表达与损伤时间的关系
- Author:
Wei LI
;
Lin QI
- Publication Type:Journal Article
- Keywords:
forensic pathology;
cerebral injury;
erythropoietin;
erythropoietin receptors;
injury tim e;
rats
- From:
Journal of Forensic Medicine
2016;32(2):90-93
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the relationship betw een injury age and expressions of erythropoietin (EPO ) and its receptor EPO R in the brain tissue of rats after cerebral injury. Methods Seventy-tw o rats w ere random ly divided into control group (36 rats) and cerebral injury group (36 rats). The rats w ere sac-rificed at 1, 2, 4, 8, 12, 24 h after cerebral injury (6 rats at each tim e point) and the brain tissues w ere extracted. The expressions of m RNA and protein of EPO and EPO R at different tim e points w ere de-tected by real-tim e fluorescent quantitative PC R and W estern bloting. Results The expressions of EPO and EPO R increased w ithin 24 h after injury. The expressions of m RNA and protein of EPO w ere relat-ed to the injury age, and the correlations w ere 0.875, 0.911, respectively (P<0.05). The expressions of m RNA and protein of EPO R w ere related to the injury age, and the correlation coefficients w ere 0.936, 0.905, respectively (P<0.05). Conclusion The expressions of EPO and EPO R increase gradually in the early stage of the rat’s cerebral injury, w hich are associated w ith the injury age and could be a useful value for estim ating injury age.
