Establishment and Verification of 6-color Fluorescent-labeled Rapid PCR Amplifi-cation System
10.3969/j.issn.1004-5619.2016.02.008
- VernacularTitle:六色荧光标记快速PCR扩增体系的建立及验证
- Author:
Yaju LIU
;
Juntao ZHANG
;
Haiying JIN
;
Meisen SHI
- Publication Type:Journal Article
- Keywords:
forensic genetics;
short tandem repeat;
rapid PCR;
multiplex STR detection
- From:
Journal of Forensic Medicine
2016;32(2):109-113
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish the rapid PCR am plification program and system and to verify the technical indexes. Methods PCR m ultiplex and capillary electrophoresis detection of 24 autosom al STR loci and one Y-STR loci using the 6-color fluorescence m arking technology, as w ell as Amelogenin and Y-InDel. Meanw hile, sensitivity, specificity, identity, stability, m ixing and a batch of sam ple tests w ere investigated, and the genotype of various routine sam ples and degraded, exfoliated cell sam ples w ere observed. Results The sensitivity of the system w as 0.062 5 ng. In addition, the genotype could be detected accu-rately only around 65 m in via rapid am plification. The species-specificity w as high and the genotyping of all kinds of dry blood specim ens of filter paper and m ixed, degraded, exfoliated cell sam ples w ere accu-rate. Conclusion The rapid am plification system can significantly im prove the detection rate, and obtain accurate and stable genotyping results, w hich m ay be im portant im plications for the establishm ent of STR database and study on population genetics and forensic identification.
