Comparison of Different Pretreatment Methods for DNA Extraction from Teeth
10.3969/j.issn.1004-5619.2016.02.011
- VernacularTitle:不同前处理方法提取牙齿DNA的比较
- Author:
Jihuai LUO
;
Hongbing SUN
;
Xin YANG
;
Zilong ZHANG
;
Tao WANG
- Publication Type:Journal Article
- Keywords:
forensic genetics;
D N A;
tooth;
extraction;
pretreatm ent
- From:
Journal of Forensic Medicine
2016;32(2):123-125
- CountryChina
- Language:Chinese
-
Abstract:
Objective To com pare the concentration of teeth DNA extracted by three different pretreatm ent m ethods and to explore a sim ple, econom ical and practical pretreatm ent m ethod w ith high concentration of extracted DNA from teeth. Methods A total num ber of 21 m olars w ere collected from 7 corpses. The pretreatm ent of 3 m olars from each individual w as random ly perform ed by tooth crum b m ethod, ball-m illing m ethod and liquid nitrogen m illing m ethod and 50 m g tooth crum b w as w eight and DNA w as extracted by A utoM ate ExpressTM forensic DNA extraction system . Subsequently, the concentration of DNA and corresponding STR genotyping of three m ethods w ere com pared. Results The DNA concentration extracted by tooth crum b m ethod, ball-m illing m ethod and liquid nitrogen m illing m ethod w as 0.055 6-1.989 1 ng/μL , 0.036 6-1.175 6 ng/μL and 0.037 8-1.249 0 ng/μL , respectively. The DNA concentration ob-tained by tooth crum b m ethod w as higher (P<0.05) and the success rate of STR genotyping w as high. Conclusion C om bined w ith A utoM ate ExpressTM forensic DNA extraction system , tooth crum b m ethod is an efficient and feasible m ethod to extract DNA from teeth, w hich can be applied in forensic practice.
