Effects of minocycline on the cognition and expression of BDNF, Bcl-2 and Bax in hippocampus of Alzheimer’s disease model rats
10.11958/20160028
- VernacularTitle:美满霉素对阿尔茨海默病大鼠认知损伤及海马BDNF、Bcl-2和Bax表达的影响
- Author:
Manli SUN
;
Haifeng DENG
;
Minghua LI
;
Guoliang LIU
;
Quanzhong CHANG
- Publication Type:Journal Article
- Keywords:
Tetracyclines;
Alzheimer disease;
apoptosis;
nerve tissue proteins;
cognition;
Minocycline;
brain-derived neurotrophic factor
- From:
Tianjin Medical Journal
2016;44(9):1088-1091
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of minocycline on the cognition and expressions of brain-derived neurotrophic factor (BDNF), apoptosis related factor Bcl-2 and Bax in hippocampus of rats with Alzheimer’s disease (AD).
Methods The rat model was established by microinjection of Aβ25-35 into lateral ventricle. Thirty healthy male SD rats were randomly divided into three groups:control group, model group and minocycline treatment group. Normal saline 1 mL/(kg·d) was intraperitoneally injected in control group and model group. The minocycline treatment group was intraperitoneally injected with minocycline 50 mg/(kg · d) for 14 days. Morris water maze was used to detect the behaviors of animals. The expressions of BDNF, Bcl-2 and Bax in hippocampus were measured by Western blotting and enzyme linked immunosorbent assay (ELISA). The apoptosis of neurons was detected by TdT-mediated dUTP nick-end labeling (TUNEL). Results Minocycline greatly improved the behaviors of AD rats, up-regulated the expressions of BDNF and Bcl-2, and down-regulated the expression of Bax in hippocampus, and reduced cell apoptosis. Conclusion Minocycline plays a protective role in neural function by promoting the growth of neurons and inhibiting the neuronal apoptosis.
