Effect of puerarin on cholesterol influx and efflux in RAW264.7-treated foam cells
10.3969/j.issn.1000-4718.2016.09.014
- VernacularTitle:葛根素对 RAW264．7源性泡沫细胞胆固醇摄取及外排功能的影响
- Author:
Zhuhua WEN
;
Dawei FU
;
Jing WANG
;
Ruqing XU
;
Gongxiong WU
;
Shiming LIU
- Publication Type:Journal Article
- Keywords:
Puerarin;
Foam cells;
Cholesterol metabolism;
Autophagy
- From:
Chinese Journal of Pathophysiology
2016;32(9):1614-1620
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To study the protective effect of puerarin on the atherosclerosis of RAW264.7-derived foam cells.METHODS: The model of foam cells was established by incubating the RAW264.7 cells with ox-LDL.The choles-terol uptake was evaluated by a DiI-ox-LDL binding assay.The ability of cholesterol efflux of the RAW264.7-derived foam cells was detected by cholesterol efflux assay.The protein levels of LC3II, P62, CD36, ABCA1, LAL and p-AMPK were determined by Western blot.RESULTS: Puerarin treatment reduced the cholesterol uptake capacity and enhanced the cho-lesterol efflux rate.The protein levels of LC3II, ABCA1 and LAL in puerarin group were higher than that in ox-LDL group, while the protein levels of P62 and CD36 were obviously decreased, and those in rapamycin treatment group had the same change as puerarin group.The protein levels of LC3II, ABCA1 and LAL were obviously decreased and the protein level of p-AMPK was increased after co-treated with 3-MA.CONCLUSION: Puerarin promotes LAL and ABCA1-mediated cho-lesterol efflux in ox-LDL-treated RAW264.7 macrophages, which might enhance autophagy through AMPK-dependent path-way for cholesterol efflux regulation, and reduce the uptake of lipids by CD36 negative regulation.
