KSHV RTA upregulates expression of survivin through interacting with GC/Sp1 and p53 cis-acting elements
10.3969/j.issn.1000-4718.2016.09.022
- VernacularTitle:KSHV RTA 通过 GC/Sp1和 p53顺式元件上调 survivin 表达
- Author:
Jianming GAO
;
Srobertson ERLE
- Publication Type:Journal Article
- Keywords:
Kaposi’s sarcoma-associated herpesvirus;
Replication and transcription activator;
Survivin;
GC/Sp1;
p53;
Promoter activity
- From:
Chinese Journal of Pathophysiology
2016;32(9):1666-1669
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To identify the potential elements within the survivin promoter indispensable for the upregu-lation of survivin by Kaposi’ s sarcoma-associated herpesvirus encoded replication and transcription activator ( KSHV RTA).METHODS: A series of truncated survivin promoter luciferase constructs were generated.Reporter assay and chromatin immunoprecipitation were performed to detect the interaction between RTA and the important cis-acting elements. RESULTS: Deletion of the GC/Sp1 and p53 binding sites within the survivin promoter almost completely shut down the survivin promoter activity and the p53 cis-acting element synergistically contributes to survivin promoter activation by RTA. CONCLUSION: KSHV RTA interacts with the GC/Sp1 and p53 cis-acting elements and regulates the expression of cellu-lar survivin by specifically increasing the activity of survivin promoter.
