Effect of somatostatin analogue octreotide on metabolism of LPS-induced A549 cells
10.3969/j.issn.1000-4718.2016.09.027
- VernacularTitle:奥曲肽对 LPS 处理的 A549细胞代谢的影响
- Author:
Luoyang RUAN
;
Xiaohong XU
;
Fengjuan PAN
;
Cuiming ZHONG
;
Xiaohua TIAN
;
Cailan YANG
;
Yalan LI
- Publication Type:Journal Article
- Keywords:
A549 cells;
Octreotide;
Lipopolysaccharides;
Metabolism
- From:
Chinese Journal of Pathophysiology
2016;32(9):1694-1699
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the effects of octreotide on metabolism in the A549 cells treated with lipopo-lysaccharides ( LPS).METHODS: The technologies of gas chromatography/mass spectrometry (GC/MS) and liquid chromatography/mass spectrometry (LC/MS) were used to test the metabolism of lung A549 cells subject to different treat-ment with LPS and/or octreotide.The results were visualized and checked by chromatogram, and the corresponding intensi-ty data were analyzed by the principal component analysis(PCA)method.The metabolites with different expression and the underlying interaction network were resolved.RESULTS: The metabolism analysis by LC/MS method indicated that there were different expression levels between different treated groups.Further analysis was carried out by orthogonal projections to latent structures-discriminant analysis (OPLS-DA) and the different expressed metabolites were obtained, which were mainly amino acids and phospholipids.By analyzing with GC/MS method and t-test, the different expressed metabolites were mainly organic acid, saccharides and amino acid metabolite.The interaction network diagram was constructed about the response of A549 cells induced by LPS and/or octreotide, including glycolysis/gluconenogenesis, tryptophan metabo-lism, galactose metabolism, urine cycle and citrate cycle.Fourteen key components were found such as serotonin, indole, threonine, serine, glucose, phenylalanine, lactose, fumarate, 4-hydroxyphenyllactate, aspartate, asparagine, putrescine, proline and succinate.CONCLUSION: In octreotide treated LPS-induced A549 cells, the main metabolites are organic acid, saccharides, amino acids and phospholipids.The interaction network is constructed, including 5 metabolic pathways
and 14 key components.
