Effect of Electroacupuncture on Cartilage Expression of TypeⅠ Collagen Gene in a Rat Model of MIA-induced Knee Osteoarthritis
10.13460/j.issn.1005-0957.2016.09.1119
- VernacularTitle:电针对膝骨关节炎MIA模型大鼠软骨组织中Ⅰ型胶原蛋白基因表达的影响
- Author:
Ying SHOU
;
Miaomiao LIU
;
Junmei ZHOU
;
Sujun LIU
;
Siwei XU
;
Yang YANG
;
Kaiyong ZHANG
;
Bimeng ZHANG
- Publication Type:Journal Article
- Keywords:
Acupuncture treatment;
Electroacupuncture;
Osteoarthritis,knee joint;
Monomer sodium iodoacetate;
TypeⅠ collagen
- From:
Shanghai Journal of Acupuncture and Moxibustion
2016;35(9):1119-1121
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate whether inhibiting the expression of typeⅠ collagen gene is one of the mechanisms of action of electroacupuncture in improving knee osteoarthritis.Methods Forty male adult SD rats were randomized into normal, model, medication and electroacupuncture group, 10 rats each. A rat model of MIA-induced knee osteoarthritis was made by injecting monomer sodium iodoacetate (MIA) and driving rat movement. After model making, the medication group received an oral gavage of celecoxib dissolved in 10% DMSO and the electroacupuncture group, electroacupuncture at points Zusanli and Yanglingquan. Pain thresholds and the levels of cartilage expression of typeⅠ collagen mRNA were compared between various groups of rats before and after treatment.Results There was a statistically significant difference in pain threshold between the model, medication or electroacupuncture group of rats and the normal group after model making (P<0.01) and between the medication or electroacupuncture group of rats and the model group before sacrifice (P<0.01). There was no statistically significant difference in pain threshold between the medication and andelectroacupuncture groups of rats before sacrifice (P>0.05). There was a statistically significant difference in the expression of typeⅠ collagen mRNA between the model, medication or electroacupuncture group of rats and the normal group (P<0.01) and between the medication or electroacupuncture group of rats and the model group before sacrifice (P<0.01). There was no statistically significant difference in the expression of typeⅠ collagen mRNA between the electroacupuncture and medication groups of rats before sacrifice (P>0.05).Conclusion The mechanisms of actions of electroacupuncture and medication in treating knee osteoarthritis may be related to inhibiting the expression of typeⅠ collagen mRNA.
