Study of genetic polymorphisms of miR-146a,miR-149 gene in Guangxi popula-tion
10.3969/j.issn.1000-484X.2016.09.008
- VernacularTitle:中国广西人群miR-146 a、miR-149基因遗传多态性的研究
- Author:
Hongcheng LUO
;
Chunfang WANG
;
Ming LEI
;
Ying WEI
;
Tan TAN
;
Yesheng WEI
- Publication Type:Journal Article
- Keywords:
miR-146a;
miR-149;
SNP;
Ethnic
- From:
Chinese Journal of Immunology
2016;32(9):1282-1285
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the frequencies of allele and genotype distribution of miR-146a C>G(rs2910164) and miR-149 T>C( rs2292832) gene, and to analyze the statistical differences between different racial and nationalities.Methods:The Polymerase Chain Reaction-Single Base Extension ( PCR-SBE) technique and DNA sequencing methods were used for the determination of the SNP in miR-146a C>G and miR-149 T>C gene,and compared with the European, African, Japanese and People in Beijing from the Human Genome Project (HapMap).Results:There were no statistical differences of allele and genotype distribution in miR-146a C>G,miR-149 T>C between female and male group (P>0.05).There were significant difference frequencies of allele and genotype distribution of miR-146a C>G and miR-149 T>C gene by compared with the European, African and People in Beijing( P<0.05).Conclusion:There were gene Polymorphisms of miR-146a C>G and miR-149 T>C in Guangxi populations, and there were significant differences by compared with other ethnic populations, which may play an important role in the human inherited disease research.
