Expression and significance of Rac1 and WAVE2 in glomerulus of high-fat diet induced C57BL/6J model mice
10.11958/20150315
- VernacularTitle:Rac1和WAVE2蛋白在高脂饮食C57BL/6J幼鼠肾小球中的表达及意义
- Author:
Yongyu ZHEN
;
Hao AI
;
Xiaoming LI
- Publication Type:Journal Article
- Keywords:
kidney glomerulus;
rac1 GTP-binding protein;
Wiskott-Aldrich syndrome protein;
immunohistochemistry;
blotting,Western;
mice,inbred C57BL;
high-fat diet;
WAVE2
- From:
Tianjin Medical Journal
2016;44(9):1081-1083
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the expression and significance of Rac1 and WAVE2 protein in glomerulus of high-
fat diet induced C57BL/6J model mice. Methods Thirty-two male C57BL/6J mice (3-week old) were randomly assigned into two groups(16 in each group). The control group was fed with basic diet (10%fat) for 4 weeks. The high-fat diet group was fed with high-fat diet (60%fat) for 4 weeks. The kidney morphological changes were examined by HE and PAS staining. The expressions of Rac1 and WAVE2 protein were examined by Western blot and immunohistochemistry analysis. Results HE and PAS results showed that there were glomeruli mesangial matrix hyperplasia and exudation in high-fat diet group compared with control group. The immunohistochemistry and Western blotting results showed that expressions of Rac 1 and WAVE2 in glomerulus were both increased in high-fat diet group compared with those of control group. Conclusion Rac1 and WAVE2 protein may be involved in glomerular injuries induced by high-fat diet.
