Analysis of microRNA expression profile of peripheral Treg cells in ITP

VernacularTitle:免疫性血小板减少症患者外周血Treg细胞microRNA分析
Author: Yan ZENG ; Hong HAN ; Hui CHEN
Publication Type:Journal Article
Keywords: Immune thrombocytopenia; Treg cells; microRNA
From: Chinese Journal of Immunology 2016;32(9):1350-1353
CountryChina
Language:Chinese
Abstract: Objective:To determine whether there were alteration of miRNA expression in peripheral Treg cells,through the contrast between normal people and patients with ITP and to explore the pathogenesis of ITP.Methods:Peripheral blood was obtained from ITP patients and normal people ,and Treg cells were isolated by flow cytometry.RNA was extracted from Tregs in each group,then for miRNA arrays analysis through Solexa sequencing.Compare ITP group and normal control group microarray to identify differentially expressed miRNA.Differentially expressed miRNA were analyzed to find the abnormal signaling pathways.Results: We screened multiple miRNA abnormal expression in Treg cell of ITP patients,which has significant differences were miR-1976,miR-548ae-5p,miR-5096-p3,miR-548am-5p,PC-3p-63471,and PC-3p-96627.By enrichment analysis,we found two abnormal signaling pathways:ErbB signaling pathway and TGF-βsignaling pathway.Conclusion:Differentially expressed miRNA were found in peripheral Treg cell of ITP patients,which may be affected the normal functioning immune regulation of Treg cells,and involved in the pathogenesis of ITP.