Construction of shRNA targeting AGR2 gene and effect of AGR2 on bio-logical function of nasopharyngeal carcinoma cells
10.3969/j.issn.1000-4718.2016.09.002
- VernacularTitle:前梯度蛋白2干扰质粒的构建及其对鼻咽癌细胞生物学功能的影响
- Author:
Yuejin LI
;
Weiwei WANG
;
Jinping LU
;
Faqing TANG
- Publication Type:Journal Article
- Keywords:
Nasopharyngeal carcinoma;
Anterior gradient protein 2;
Neoplasm metastasis
- From:
Chinese Journal of Pathophysiology
2016;32(9):1545-1550
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To construct the shRNA targeting anterior gradient protein 2 (AGR2) gene for exploring the effect of AGR2 on the biological behavior of nasopharyngeal carcinoma (NPC) cells.METHODS: The expression of AGR2 at mRNA and protein levels in NPC cell lines 6-10B and 5-8F was detected by real-time PCR and Western blot.The pSR-GFP/Neo-AGR2-shRNA expression vector targeting AGR2 was constructed.Based on the interference targeting AGR2, the cell migration and motility were determined by Transwell migration and motility assays.RESULTS: The expression of AGR2 was increased in NPC cell line 5-8F compared with NPC cell line 6-10B (P <0.05).When the AGR2 expression in 5-8F cells was interfered, the cell migration, invasion and tumorigenicity were weakened.CONCLUSION: The expres-sion of AGR2 is up-regulated in NPC cell line 5-8F.pSR-GFP/Neo-CLU-shRNA successfully inhibits the expression of AGR2 in NPC cell line 5-8F.AGR2 inhibits the migration, invasion and tumorigenicity of 5-8F cells in vivo.
