Pseudolaric acid B induces glioblastoma cell line U87 mitotic arrest and apoptosis
10.3969/j.issn.1000-4718.2016.09.007
- VernacularTitle:土槿皮乙酸诱导脑胶质瘤细胞株U87阻滞于M期并发生凋亡
- Author:
Lu HE
;
Chuangyu WEN
;
Huihui WANG
;
Xiangling YANG
;
Huanliang LIU
;
Zhong LI
- Publication Type:Journal Article
- Keywords:
Glioblastoma;
Pseudolaric acid B;
Cell cycle;
Cell viability;
Apoptosis
- From:
Chinese Journal of Pathophysiology
2016;32(9):1574-1578
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the effects of pseudolaric acid B on the growth and apoptosis of glioblastoma cell line U87.METHODS: The cell morphological changes were observed under inverted microscope.The cell viability was evaluated by MTS assay.The cell cycle was analyzed by flow cytometry and Western blot.The cell apoptosis was de-tected by flow cytometry.The changes of apoptosis-related proteins cleaved PARP, caspase-3, procaspase-9 and caspase-8 were determined by Western blot.RESULTS: Pseudolaric acid B inhibited the viability of U87 cells, arrested U87 cells in mitosis.Apoptosis of U87 cells was induced by pseudolaric acid B.The caspase pathway was activated.CONCLUSION:Pseudolaric acid B induces glioblastoma cell line U87 mitotic arrest and apoptosis.
