Effects of thyroid hormone receptor β△ on apoptosis and proliferation of hepatoma RH-35 cells

VernacularTitle:甲状腺激素受体β△对大鼠肝癌细胞 RH-35凋亡和增殖的影响
Author: Ronglan ZHAO ; Wei SONG ; Yanli SUN ; Qian LI ; Meng LI ; Hairong CHU ; Xiaoxiang PENG
Publication Type:Journal Article
Keywords: Thyroid hormone receptor; TRβ△ isoform; RH-35 cells
From: Chinese Journal of Endocrinology and Metabolism 2016;32(8):691-695
CountryChina
Language:Chinese
Abstract: Objective To study the effects of TRβ△ on apoptosis and proliferation of liver cancer cell line RH-35 from rat in vitro. Methods RH-35 cells were transfected by empty vector pcDNA3. 1 and expression plasmid pcDNA3. 1-TRβ△, then exposure to 10 nmol/ L T3 . RH-35 cells apoptosis and proliferation were observed by flow cytometry and MTT colorimetric assay; Levels of catenin β-1(CTNNB1), senescence marker protein-30(SMP-30) and BCL2-antagonist/ killer ( BAK ) mRNA evaluation were detected by quantitative real-time RT-PCR (RT-qPCR). Results In the presence of T3 , overexpression of TRβ△ significantly inhibited the proliferation, increased the percentage of apoptotic, down-regulated CTNNB1and SMP-30 expression, up-regulated BAK expression in RH-35 cells( P < 0. 05). Conclusion TRβ△ could inhibit the proliferation of RH-35 cells and promote their apoptosis, which may be related to upregulation of BAK genes expression and downregulation of CTNNB1 and SMP-30 gene expression, and these effects could be regulated by T3 .