A study on the expression of CD163 in neonatal rats with Escherichia coli sepsis
10.3969/j.issn.1673-6710.2016.05.014
- VernacularTitle:大肠埃希菌致脓毒症新生大鼠巨噬细胞及血中CD163表达研究
- Author:
Xinxian GUAN
;
Shenglin YU
;
Jian WANG
;
Shasha GAO
;
Yiping LI
- Publication Type:Journal Article
- Keywords:
Escherichia;
Sepsis;
Macrophages;
CD163;
Rats
- From:Chinese Journal of Neonatology
2016;11(5):375-378
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the expression of CD163 in macrophages and sCD163 level in the serum of neonatal rat model of Escherichia coli (E. coli) sepsis. Methods A total of 72 specific-pathogen-free (SPF) neonatal rats (P7) were randomly and equally assigned into experiment group and control group. E. coli was injected peritoneally and the sepsis model was established in the experiential group while normal saline (NS) was injected in the control group. Samples were collected at 2, 4, 6, 12, 24 h and 48 h after the treatment. CD163 expression in macrophages of lung and liver tissues were tested using immunohistochemical(IHC) method, and the dynamic changes of sCD163 concentration in the serum were monitored usingenzyme-linked immunosorbent assay (ELISA) method. Results In the experiment group, CD163 expression in macrophages of lung and liver were gradually decreased at eachtime point (P <0. 001). At 2 h, CD163 expression in macrophages showed no significant differences between the two groups (P >0. 05). At 4 h and later timepoints, the differences were statistically significant (P <0. 001) . Meanwhile, sCD163 in the serum increased gradually (P <0. 01). At 2 h, sCD163 in the serum showed no significant differences between the two groups (P >0. 05). At 4 h and later timepoints, the differences were statistically significant (P <0. 001). Conclusions CD163 plays an important role in sepsis.
