Comparative Analysis on Simultaneous Determination of α-pinene, β-pinene, Eucalyptol and Linalool in Baeckea Frutescens Preparations by Reference Substances Method and Reference Extract Method
10.3969/j.issn.1005-5304.2016.10.023
- VernacularTitle:对照品法和对照提取物法测定岗松油中α-蒎烯、β-蒎烯、桉油精和芳樟醇含量比较分析
- Author:
Yi LUO
;
Guichang BAI
;
Minyi LU
- Publication Type:Journal Article
- Keywords:
reference substances method;
reference extract method;
gas phase chromatography;
Baeckea frutescens;
α-pinene;
β-pinene;
eucalyptol;
linalool;
content determination
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2016;23(10):100-103
- CountryChina
- Language:Chinese
-
Abstract:
Objective To determinate the contents of α-pinene, β-pinene, eucalyptol and linalool in Baeckea frutescens by reference substances method and reference extract method respectively; To explore the feasibility of replacing single component reference by control extracts in assay. Methods The GC system consisted of a quartz column DB-5 (60 m×0.25 mm×0.25 μm); The temperature programming rose from 80 ℃ (15 min) to 90 ℃ by 1 ℃/min, lasting 2 min, then 10 ℃/min to 110 ℃, then 25 ℃/min to 240 ℃, lasting 8 min in the end; The temperature of the entrance of capillary vessel column was 250 ℃, and the temperature of the detector was 250 ℃. Results α-pinene, β-pinene, eucalyptol and linalool were in a good linear relationship within each concentration scope (r≥0.999). The average recovery rates were in the range of 96.5%–102.2%. The results of t-test demonstrated that there is no significant difference between the two methods. Conclusion The reference extract method can be used as a quality evaluation pattern for Baeckea frutescens.
