Determination of heterophyllin B inTaizishen-Huangqi oral solution by HPLC
10.3760/cma.j.issn.1673-4246.2016.09.020
- VernacularTitle:HPLC法测定太子参黄芪口服液中太子参环肽B的含量
- Author:
Yongqin PANG
;
Jinghong QU
- Publication Type:Journal Article
- Keywords:
Heterophyllin B;
Taizishen-Huangqi oral solution;
Quality control;
High performance liquid chromatography
- From:
International Journal of Traditional Chinese Medicine
2016;38(9):841-843
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study a method for the determination of heterophyllin B inTaizishen- Huangqi oral solution.Methods The HPLC system consisted of Phenomenex Luna-C18 (4.6 mm × 250 mm, 5μm) column, the mobile phase consisted of acetonitrile and 0.1% acetic acid, the gradient elution flow rate was 1.0 ml/min, the column temperature was 30℃, and the UV detector was set at 203 nm.Results The linear response range was 0.016-0.320 mg/ml (r=0.999 8). The average recovery of heterophyllin B was 98.92%, and RSD was 0.80%. The average determination of Heterophyllin B within 5 batches of preparation was 1.63 mg/ml. Conclusions The method showed its high sensitivity, accurate, repeatable and high secificity. It could be used as a method to control the standard ofTaizishen-Huangqi oral solution.
