Expression and clinical signiifcance of sB7-H3 and cytokines in the bronchoalveolar lavage lfuid in children with refractory Mycoplasma pneumoniae pneumonia
10.3969/j.issn.1000-3606.2016.08.001
- VernacularTitle:难治性肺炎支原体肺炎患儿肺泡灌洗液中sB7-H3及细胞因子表达
- Author:
Xinxing ZHANG
;
Zhengrong CHEN
;
Wenjing GU
;
Yongdong YAN
;
Yuqing WANG
;
Canhong ZHU
;
Li HUANG
;
Meijuan WANG
;
Xuejun SHAO
;
Wei JI
- Publication Type:Journal Article
- Keywords:
refractory Mycoplasma pneumoniae pneumonia;
bronchoalveolar lavage lfuid;
sB7-H3;
cytokines;
child
- From:
Journal of Clinical Pediatrics
2016;34(8):561-565
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the level of expression, clinical signiifcance of sB 7-H 3 in the bronchoalveolar lavage lfuid (BALF) of refractory Mycoplasma pneumoniae (MP) pneumonia (RMPP) in children and the relationship between sB7-H3 and various cytokines. Methods The BALF of forty-three hospitalized children with RMPP (RMPP group) were collected for the diagnosis and treatment. Thirteen cases were lavaged only once and the other thirty cases had collected the BALF twice. The BALF of iffteen hospitalized children with bronchial foreign body were collected as control group. The expression levels of sB 7-H 3 , IL-1β, IL-2 and IL-36 in the BALF were detected by enzyme-linked immunosorbent assay. The expression levels of sB 7-H 3 , IL-1β, IL-2 and IL-36 in the BALF at the acute phase were compared with control group and the group after treatment. Analyzed the correlation between the level of sB 7-H 3 and IL-1β, IL-2 , IL-36 in the BALF of RMPP children at acute stage. Results The levels of sB 7-H 3 , IL-1β and IL-36 in the BALF of the ifrst lavage group were higher than those of single lavage group and control group (all P<0 . 05 ). The levels of sB 7-H 3 , IL-1β, IL-2 and IL-36 in the BALF of single lavage group were higher than those of control group (all P<0 . 05 ). The levels of sB 7-H 3 , IL-1β, IL-2 and IL-36 in the BALF of the second lavage group were lower than those of the ifrst lavage group (all P<0 . 05 ).The levels of sB 7-H 3 , IL-2 in the BALF of the second lavage group were higher than those in the control group (both P<0 . 05 ), but the levels of IL-1β, IL-36 in the BALF showed no difference between the second lavage group and the control group (both P>0 . 05 ). The levels of sB 7-H 3 had positive correlation with the levels of IL-1β, IL-2 and IL-36 (all P<0 . 001 ). Conclusions sB 7-H 3 may control the secretion of IL-1β, IL-2 and IL-36 , and participate in immune response and lung injury after MP infection, which may lead to occurrence and development of RMPP.
