Effects of Sishen Pills on Expressions of TLR-2 and TLR-4 of Colonic Tissue in Rats with Ulcerative Colitis
10.3969/j.issn.1005-5304.2016.10.016
- VernacularTitle:四神丸对溃疡性结肠炎模型大鼠结肠组织Toll 样受体2、4表达的影响
- Author:
Xiangdong ZHU
;
Yan WANG
;
Lanjuan HE
;
Tingting GUO
;
Di WANG
- Publication Type:Journal Article
- Keywords:
ulcerative colitis;
Sishen Pills;
Toll-like receptor 2;
Toll-like receptor 4;
rats
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2016;23(10):67-71
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effects of Sishen Pills on gene and protein expressions of toll-like receptor 2 (TLR-2) and toll-like receptor 4 (TLR-4) of colonic tissue in spleen-kidney yang deficiency rats with ulcerative colitis (UC); To discuss its mechanism of action for spleen-kidney yang deficiency UC. Methods Lavage of senna +intraperitoneal injection of hydrocortisone injection + enema of 2,4,6-trinitrobenzene sulfonic acid/ethanol were used to establish the model of UC of spleen-kidney yang deficiency type. 90 Wister rats were randomly divided into blank group, model group, Salazosulfadimidine group and the Sishen Pills high-, medium- and low-group. Each medication group was intervened by relevant medicine. RT-qPCR was used to detect the expressions of TLR-2 and TLR-4, and Western blot was used to detect the protein expressions of TLR-2 and TLR-4. SOD and MDA in serum were detected. Results Compared with the blank group, SOD activity decreased and MDA content increased in the model group (P<0.01); gene and protein expressions of TLR-2 and TLR-4 increased significantly (P<0.05). Compared with model group, SOD increased and MDA content decreased in all the medication groups (P<0.05, P<0.01); gene and protein expressions of TLR-2 and TLR-4 decreased (P<0.05, P<0.01). Conclusion Sishen Pills can achieve the treatment of UC by improving the metabolism of oxygen radicals in colonic tissue and regulating the immune system of the intestinal epithelium.
