Effects ofβ-Elemene on Apoptosis of Human Pancreatic Cancer Panc-1 Cells
10.3969/j.issn.1005-5304.2016.10.018
- VernacularTitle:β-榄香烯对人胰腺癌 Panc-1细胞凋亡的影响
- Author:
Bingjun WANG
;
Xiankun WANG
;
Bo YAN
;
Shaoping LI
- Publication Type:Journal Article
- Keywords:
β-Elemene;
pancreatic cancer;
Panc-1 cells;
cell apoptosis
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2016;23(10):78-81
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of β-Elemene on the apoptosis of human pancreatic cancer Panc-1 cells; To discuss its mechanism of action. Methods β-Elemene (10, 20, 40, 80, 160 μg/mL) were incubated to the Panc-1 cells in vitro cultured for 24 h, 48 h and 72h, and trypan blue refusal method was used to detect cell inhibition rate;Apoptosis rate was measured by TUNEL; Hoechst33258 fluorescent staining was used to observe the changes of the nucleus. The activity of Caspase-3, 8 and 9 were detected by ELISA. Western blot was used to detect the expressions of Fas, FasL and Cyt c and AIF. Results The activity of Panc-1 cells was obviously inhibited time/concentration dependent inhibition (P<0.05, P<0.01), and the apoptosis rate increased after incubated with β-Elemene (P<0.01, P<0.001) after incubated with β-Elemene for 24 h, 48 h and 72 h; After giving β-Elemene 72 h, Panc-1 cells nucleus were broken obviously, and chromatin condensed and showed strong blue fluorescence, along with of apoptotic bodies; After incubated with β-Elemene for 48 h, Caspase-3, 8 and 9 activity significantly increased (P<0.05, P<0.01); protein expressions of Fas, FasL, Cyt c and AIF were significantly enhanced (P<0.05, P<0.01, P<0.001). Conclusion β-Elemene can inhibit Panc-1 cell proliferation, induce apoptosis, and the mechanism may be related to activating cell death receptor pathway and mitochondrial apoptosis pathway to play anti-tumor effects.
