Content Determination of Hyodeoxycholic Acid in Artificial Calculus Bovis by Pre-Column Derivatization HPLC-UV Method
10.3969/j.issn.1005-5304.2016.10.021
- VernacularTitle:柱前衍生 HPLC-UV 法测定人工牛黄中猪去氧胆酸含量
- Author:
Huan YANG
;
Yuezhu DING
;
Tianxuan DUAN
;
Yu FU
;
Xiongfei WANG
;
Zhaoyi WANG
;
Ruijuan YUAN
- Publication Type:Journal Article
- Keywords:
artificial calculus bovis;
hyodeoxycholic acid;
pre-column derivation;
HPLC;
UV
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2016;23(10):92-94
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a pre-column derivation HPLC-UV method for the content determination of hyodeoxycholic acid in artificial calculus bovis. Methods The hyodeoxycholic acid was derived by 2-bromo-2’-acetonaphthoneat using triethylamine as the catalyst in 60 ℃ water bath. After that, a HPLC-UV method was established to determine the content of hyodeoxycholic acid in artificial calculus bovis. Results When the derivatising time at 60 ℃ water bath was 50 min, the radio of the molar amount of derived reagents and hyodeoxycholic was over 20:1 and the radio of catalyst and hyodeoxycholic was over 15:1; the reaction was completed. The calibration curve was linear within the range of 0.1–2 μg for hyodeoxycholic acid (r=0.999 7), and the average recovery was 97.85% (RSD=1.6%). In this sample, the content of hyodeoxycholic is 4.12%. Conclusion The method is with high sensitivity, highly reproducible, reliable and accurate for the content determination of hyodeoxycholic acid in artificial calculus bovis.
