Tumor necrosis factor-alpha pretreated umbilical cord blood mesenchymal stem cell transplantation for treatment of myocardial infarction
10.3969/j.issn.2095-4344.2016.32.018
- VernacularTitle:肿瘤坏死因子α预处理脐血间充质干细胞移植治疗心肌梗死
- Author:
Wei WANG
;
Xiaofu LI
;
Zhongjian LI
- Publication Type:Journal Article
- From:
Chinese Journal of Tissue Engineering Research
2016;20(32):4831-4837
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:A large amount of inflammatory mediators from myocardial cels are secreted in response to myocardial injury after myocardial ischemia. Moreover, inflammatory cytokines in infarction and ischemia regions contribute to myocardial tissue repair and adaptation.
OBJECTIVE:To investigate the effect of tumor necrosis factor-α (TNF-α) pretreatment on cardiac function of myocardial infarction rabbits undergoing umbilical cord blood mesenchymal stem cel transplantation.
METHODS: Thirty-six white rabbits were equally randomized into sham, model, non-TNF-α, and TNF-αgroups. Animal models of myocardial infarction were made in the latter three groups. Twenty-four hours after modeling, PBS, umbilical cord blood mesenchymal stem cells pretreated with or without TNF-α were injected at infarct center and border, respectively, in the model, TNF-α and non-TNF-α groups.
RESULTS AND CONCLUSION: Model rabbits in the TNF-α and non-TNF-α groups showed better cardiac function and lower size of myocardial infarction and fibrosis than those in the model group. Compared with the non-TNF-α group, moreover, the TNF-α group showed better outcomes in these indicators. These findings indicate that TNF-α pretreatment canmarkedly improve the therapeutic efficacy of umbilical cord blood mesenchymal stem cel transplantation on myocardial infarction.
