Determination of content and related substances of radiosensitizer benzotriazine derivatives YABQ by HPLC
10.7644/j.issn.1674-9960.2016.08.005
- VernacularTitle:HPLC法测定放射增敏剂苯并三嗪衍生物YABQ含量及有关物质
- Author:
Shaolei LIU
;
Jianyun YANG
;
Bingkun XIAO
;
Rongqing HUANG
- Publication Type:Journal Article
- Keywords:
quantitative analysis;
radiation-sensitizing agents;
YABQ;
quality control;
HPLC;
benzotriazine
- From:
Military Medical Sciences
2016;40(8):639-642
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish an HPLC method for determination of the content of radiosensitizer YABQ and its related substances.Methods Diamonsil C18 column (250 mm ×4.6 mm,5 μm) was used.The mobile phase consisted of methanol-0.1%formic acid (22∶78) and isocratic elution at a flow rate of 1.0 ml/min.The detection wavelength was 266 nm,the temperature of the column was 30℃, and the injection volume was 10 μl.Results The linear range of YABQ was 7-84 μg/ml, r=0.9992.The detection limit was 8 ng (S/N≥3), and the quantitation limit was 24 ng (S/N≥10). According to destructive sample processing, the separation coefficient between the peaks was above 1.5, indicating that this chromatographic method could meet the YABQ monitoring requirements.Conclusion Method validation and destructive testing show that both the precision and specificity are fine with this method, which could be used for quality control of YABQ and its related substances.
