Bone marrow mesenchymal stem cells for repairing acute respiratory distress syndrome
10.3969/j.issn.2095-4344.2016.32.012
- VernacularTitle:骨髓间充质干细胞修复急性呼吸窘迫综合征
- Author:
Lei ZHANG
- Publication Type:Journal Article
- From:
Chinese Journal of Tissue Engineering Research
2016;20(32):4792-4797
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:To date little is reported clinicaly on bone marrow mesenchymal stem cels (BMSCs) for acute respiratory distress syndrome (ARDS).
OBJECTIVE:To study the effect of BMSCs in ARDS rats.
METHODS:Sixty-three Sprague-Dawley rats were randomly assigned into normal control group, model and BMSCs groups (n=21 per group), folowed by establishment of ARDS models. After awakening, rats in the model group were given no treatment, while those in the BMSCs group given tail vein injection of fluorouracil-labeled BMSCs. Then, pathological observation of the lung tissue was conducted using hematoxylin-eosin staining, and ELISA method was employed to detect interleukin-1β level in the rat lung tissue homogenates. Repair effects on lung injury were compared between two groups.
RESULTS AND CONCLUSION: Hematoxylin-eosin staining showed that inflammation, edema and congestion in the rat lung tissue were vanished gradualy, but no consolidation was found in the BMSCs group, while there was visible edema and congestion in the lung tissue of rats in the model group. Compared with the model group, pulmonary edema and interleukin-1β level in the lung tissue were significantly reduced in the BMSCs group (P < 0.05). Therefore, the whole bone marrow culture method is suitable to obtain BMSCs that have desired effects on ARDS in rats. In ARDS rats, due to inhalation of lipopolysaccharide, BMSCs can be directly involved in lung epithelial repair by reducing the release of inflammatory factors.
