Application of LC-MS/MS to quantitative bioanalysis of monoclonal antibodies in biological matrices
10.7644/j.issn.1674-9960.2016.08.016
- VernacularTitle:基于液质联用技术的单克隆抗体生物样品定量分析方法的应用
- Author:
Yijue WU
;
Li LI
;
Haifeng SONG
- Publication Type:Journal Article
- Keywords:
liquid chromatography coupled to tandem mass spectrometry(LC-MS/MS);
monoclonal antibody;
quanti-tation;
enzyme-linked immunosorbent assay;
ligands
- From:
Military Medical Sciences
2016;40(8):686-690
- CountryChina
- Language:Chinese
-
Abstract:
Compared to ligand-binding assay( LBA) , liquid chromatography coupled to tandem mass spectrometry( LC-MS/MS) methods could improve specificity, reduce the time of method development and enhance efficiency for quantitation of monoclonal antibodies( mAb) .This review summarizes the application of LC-MS/MS assays to the quantitation of mAb in order to facilitate the research of quantitation of mAb in drug development.
