Preparation of monoclonal antibodies against ricin toxin and development of up-converting phosphor technology-based lateral flow assay for its quantitative detection

VernacularTitle:蓖麻毒素单抗制备及上转发光免疫层析定量检测方法研究
Author: Xiaochen WANG ; Lei ZHOU ; Chongyun SUN ; Yong ZHAO ; Xinrui WANG ; Pingping ZHANG ; Ruifu YANG ; Xin MA
Publication Type:Journal Article
Keywords: ricin toxin; up-conversion phosphor nano-particles; lateral flow assay; rapid quantitative detection
From: Military Medical Sciences 2016;40(8):676-679
CountryChina
Language:Chinese
Abstract: Objective To develop an up-converting phosphor technology based lateral flow assay ( UPT-LF) to detect ricin toxin ( RT) quickly, accurately and quantitatively.Methods Ricin-monoclonal antibodies were prepared and their affinity was evaluated before four types of monoclonal antibodies with the highest titer were applied to couple with the up-converting phosphor nano-particles ( UCP-NPs) as the bio-conjugate and disperse on the analysis membrane as the test line, respectively.Following systematic optimization to establish the RT-UPT-LF strip, the sensitivity, precision, quantita-tive ability and specificity of RT-UPT-LF were evaluated.Results The detection could be accomplished within 15 min and the detection limit of the RT-UPT-LF assay could reach 0.5 ng/ml within the quantitative detection range of 0.5-1000 ng/ml.Other non-specific toxins at a concentration of 1000 ng/ml did not cause any non-specific reactions.Conclusion The developed RT-UPT-LF strip provides a new means for on-site quantitative detection of ricin toxin.