Establishment and Evaluation of BALB/c Mouse Model of Anaphylactic Asthma with ;Yin-deficiency Syndrome
10.3969/j.issn.1005-5304.2016.09.015
- VernacularTitle:BALB/c小鼠阴虚型过敏性哮喘模型的建立与评价
- Author:
Zhiwang WANG
;
Haiyan TUO
;
Yuan REN
;
Rongke LI
;
Xuefeng LIU
;
Xiaoli CHENG
- Publication Type:Journal Article
- Keywords:
anaphylactic asthma with yin-deficiency syndrome;
animal model;
BALB/c mice
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2016;23(9):61-64
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish and evaluate a BALB/c mouse model of anaphylactic asthma with yin-deficiency syndrome. Methods Ovalbumin (OVA) was injected to sensitize and was inhaled to stimulate to replicate the BALB/c mouse model of anaphylactic asthma. Thyroxin was used for gavage during late stimulation to replicate the BALB/c mouse model of asthma with yin-deficiency syndrome. On the basis of monitoring food and water intake and body weight, asthma related indexes, such as asthmatic behaviors, respiratory function, lung histopathology, were detected, and yin-deficiency syndrome related indexes, such as cAMP and cGMP, and pulmonary fluid clearance were examined. Results The BALB/c mouse model of asthma with yin-deficiency syndrome showed obvious asthma symptoms; comprehensive scores of asthmatic behaviors increased significantly;inhaling peak flow, exsufflation peak flow, and tidal volume decreased significantly; lung tissue histopathology showed obvious inflammatory response. Meanwhile, food and water intake of BALB/c mouse model of asthma with yin-deficiency syndrome increased significantly;body weight increased slowly;wet/dry ratio of lung tissue decreased;cAMP and cAMP/cGMP increased, while cGMP decreased, which showed that the mice were in the condition of yin-deficiency and yang-excess. Conclusion Combining thyroxin at the basis of sensitivity induced by OVA is a good pattern to successfully replicate the BALB/c mouse model of anaphylactic asthma with yin-deficiency syndrome.
