Comparison of Contents of Adenine and Guanosine in Peels of Three Strains of Trichosanthes kirilowii Maxim.
10.3969/j.issn.1005-5304.2016.09.021
- VernacularTitle:不同品系栝楼果皮腺嘌呤和鸟苷含量比较
- Author:
Jingping YU
;
Yongqing ZHANG
- Publication Type:Journal Article
- Keywords:
HPLC;
Trichosanthes kirilowii Maxim.;
peel;
guanosine;
adenine
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2016;23(9):87-89
- CountryChina
- Language:Chinese
-
Abstract:
Objective To compare the contents of guanosine and adenine in peels of three strains of Trichosanthes kirilowii Maxim.;To provide references for germplasm resources evaluation and breeding of the Chinese medicinal materials. Methods HPLC method was used to determine the contents of guanosine and adenine with Waters AtlantisT3-C18 column (4.6 mm×150.0 mm, 5 μm). The mobile phase was methanol and water, and the detection wavelength was 254 nm. Results The contents of guanosinein in peels of three strains of Trichosanthes kirilowii Maxim. were 0.159 4, 0.159 6, 0.134 1 mg/g, respectively;the contents of adenine were 0.097 1, 0.127 9, 0.093 4 mg/g, respectively. Conclusion There were significient differences in the contents of adenine among three strains of Trichosanthes kirilowii Maxim.. Strain Ⅱ is with higher level of guanosine and adenine, which implies that the breeding of the Chinese medicinal materals is one way to improve the quality of Trichosanthis Exocarpium.