The effect of repetitive transcranial magnetic stimulation on behaviors and hippocampal GR protein ex-pression in depression model rats
10.3969/j.issn.1002-0152.2016.07.008
- VernacularTitle:重复经颅磁刺激对抑郁模型大鼠行为学及海马区糖皮质激素受体表达的影响
- Author:
Huicong REN
;
Shina GU
;
Lin ZHAO
;
Wenqiang LI
;
Zhaohui ZHANG
- Publication Type:Journal Article
- Keywords:
Depression;
Repetitive transcranial magnetic stimulation;
Rats;
Glucocorticoid receptor
- From:
Chinese Journal of Nervous and Mental Diseases
2016;42(7):420-425
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of repetitive transcranial magnetic stimulation (rTMS) on behaviors and hippocampal glucocorticoid receptor (GR) protein expression in chronic stress depression model rats and the possible antidepressant mechanism of rTMS. Method Seventy-five male Sprague-Dawley rats were randomly divided into the blank control group (n=15) and the stress-induced group (n=60). Singly housing and chronic unpredictable mild stress (CUMS) were used to induce the depression model in stress-induced group. Forty-five CUMS rats were selected and ran?domly divided into rTMS group (receiving 10 Hz rTMS intervention for 3 weeks), sham group (receiving pseudo rTMS treatments for 3 weeks) and depression group (with no further treatment). Body weight measurements and performance in the sucrose consumption and forced swimming test (FST) were evaluated before modeling, after modeling and after inter?vention. The GR protein and GR mRNA expression level in the hippocampus were examined after intervention. Results Compared with control group, the body weight growth rate and the sugar water preference were significantly lower in stress-induced group (P<0.01), and the immobility time of FST was significantly longer (P<0.01). After the 3-week rTMS intervention, the body weight growth rate and the sugar water preference in rTMS group, which were insignificantly differ?ent from control group (P>0.05), were higher than those in sham group and depression group (P<0.01). The immobility times of FST in rTMS group and control group were shorter than sham group and depression group (P<0.01). Compared with rTMS group and control group, GR and GR mRNA expression levels in the hippocampus were significantly reduced in sham group and depression group (P<0.01). Conclusion rTMS can improve depression behavior of CUMS rats, which may be associated with upregulation of GR expression in the hippocampus.
