Effects of Decocting Rehmanniae Radix and Paeoniae Radix Alba Single and Mixed on ;Dissolving-out Quantity of Catalpol
10.3969/j.issn.1005-5304.2016.09.023
- VernacularTitle:地黄与白芍合煎及单煎对有效成分梓醇溶出量的影响
- Author:
Liping CHEN
;
Maoxing LI
;
Jun ZHOU
- Publication Type:Journal Article
- Keywords:
Rehmanniae Radix;
Paeoniae Radix alba;
catalpol;
single decoction;
mixed decoction;
HPLC
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2016;23(9):96-98
- CountryChina
- Language:Chinese
-
Abstract:
Objective To compare the content variation of catalpol between single and mixed decocting Rehmanniae Radix and Paeoniae Radix alba; To preliminarily explore the variation rules of compatibility of Rehmanniae Radix and Paeoniae Radix alba. Methods HPLC method was used with Waters Symmetry C18 column (4.6 mm×150 mm, 5 μm), flow rate of 1.0 mL/min, detection wavelength of 210 nm, column temperature at 25 ℃. The mobile phase was acetonitrile-0.01% phosphoric acid in water isocratic elution system. Results Catalpol regression equation was Y=270 054X-57 724, r=0.999 6, showing good linear range among 0.225–7.2 μg. The average recovery rate was 101.15%, RSD=0.39%. The average content of catalpol in the mixed decoction of Rehmanniae Radix and Paeoniae Radix alba was 18.66%more than the single decoction of Rehmanniae Radix. Conclusion The content of catalpol in mixed decoction is higher than the single decoction of Rehmanniae Radix and Paeoniae Radix alba. The mixed decoction is favorable for the dissolving of catalpol from the medicine. The compatibility of Rehmanniae Radix and Paeoniae Radix alba is rational.
