Study on Determination Method of Total Flavonoids in Gansu Astragali Radix and ;Hedysarum Polybotrys
10.3969/j.issn.1005-5304.2016.09.024
- VernacularTitle:甘肃黄芪和红芪总黄酮含量对比测定方法探索研究
- Author:
Ying YE
;
Qiang BAO
;
Ruihai WANG
;
Dong BAI
;
Xin XUE
;
Lishi ZHANG
;
Limei LIU
- Publication Type:Journal Article
- Keywords:
Astragali Radix;
Hedysarum Polybotrys;
total flavonoids;
ultraviolet-visible spectrophotometry;
colorimetric method;
determination
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2016;23(9):99-105
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the determination method of total flavonoids in Gansu Astragali Radix and Hedysarum Polybotrys. Methods Calycosin glycosides etc. was selected as reference substances, comparison on the difference of absorption curves was done by ultraviolet spectroscopy and colorimetric method (NaNO2-Al(NO3)3-NaOH, AlCl3, Mg(Ac)2, NaOH, phosphomolybdic acid, HCl-Mg power). Results With colorimetric method, the maximum absorption wavelength of referrence and the test was inconsistent. The absorption peak shape was also different. With UV method, Calycosin glycosides in band Ⅱ (260 nm) showed a shoulder absorption. Astragali Radix and Hedysarum Polybotrys also showed characteristic shoulder absorptions in band Ⅱ with absorption wavelength at 263 nm and 265 nm. So the sample absorption wavelength is basically the same as that of the control sample. Conclusion Colorimetries usually used for determination of total flavonoids are not suitable for the comparison determination of Gansu Astragali Radix and Hedysarum Polybotrys. It is suitable for determining the contents of total flavonoids in samples by UV spectrophotometry at the band Ⅱ, which is the characteristic absorption band of isoflavone compound.
