The expression level of MAGEA in osteosarcoma and its association with prognosis
10.3969/j.issn.1006-5725.2016.16.012
- VernacularTitle:MAGEA在骨肉瘤中的表达和预后分析
- Author:
Changye ZOU
;
Shao XU
;
Qinglian TANG
;
Zheng YANG
;
Junqiang YIN
;
Xianbiao XIE
;
Gang HUANG
;
Jin WANG
;
Jingnan SHEN
- Publication Type:Journal Article
- Keywords:
Osteosarcoma;
Microarray analysis;
Cancer-testis antigens;
Prognosis
- From:
The Journal of Practical Medicine
2016;32(16):2636-2641
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression level and clinical significance of melanoma antigen gene A (MAGEA) in osteosarcoma patients. Methods Compare gene expression profiles in osteosarcoma cell lines and osteoblasts with gene microarrays. Validation of differentially expressed genes was carried out by real-time polymerase chain reaction analysis. Corresponding protein levels were measures by Western blot analysis in osteosarcoma cell lines and by immunohistochemistry in osteosarcoma tissues. The staining intensity of immuno-histochemistry was correlated with clinical outcome , and its prognostic significance was analyzed. Results Sev-eral genes belonging to MAGEA increased significantly in all osteosarcoma cell lines and tumor tissue , but not in normal osteoblast cell. Patients with MAGEA expression has higher risk of lung metastasis (relative risk 2.79, 95% confidence interval, 1.12-6.93; P = 0.028) and lower five-year survival rates (39.6% ± 8.4% vs. 80% ± 8.9%, P = 0.01) compared with patients without MAGEA expression. Conclusions The expression of MAGEA increased in osteosarcoma , which inversely correlating with outcome of osteosarcoma patients.
