Establishment and optimization of loop-mediated isothermal amplification for rapid detection of pseu-domonas aeruginosa
10.3969/j.issn.1006-5725.2016.16.023
- VernacularTitle:应用环介导等温扩增技术对分离自呼吸系统感染铜绿假单胞菌的快速检测
- Author:
Qun LIN
;
Jieyi FENG
;
Jinhua HUANG
;
Shaomin HUANG
;
Zhigang WU
- Publication Type:Journal Article
- Keywords:
LAMP;
Visual detection;
SYBR Green;
Rapid detection;
P. aeruginosa
- From:
The Journal of Practical Medicine
2016;32(16):2677-2679
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a Loop-mediated Isothermal Amplification (LAMP) for Pseudomonas aeruginosa rapid detection. Method 152 P. aeruginosa strains isolated from nasal swabs and 30 reference strains were applied. P. aeruginosa ATCC15442 was used to develop LAMP amplification and evaluate sensitivity and specificity. Results Sensitivity of LAMP was 103 times higher than PCR, with DNA amount as 132 fg. When LAMP was applied to 30 reference strains and 152 P. aeruginosa strains , the specification was 100% while iden-tification rate reached 94.7%. Conclusion The establishment LAMP showed a promising prospect in P. aerugi-nosa rapid detection.
