The roles of spinal microglial Src-family kinases in diabetic neuropathic pain
10.3969/j.issn.1006-5725.2016.16.002
- VernacularTitle:脊髓小胶质细胞酪氨酸家族激酶在糖尿病神经痛中的作用
- Author:
Honghua WANG
;
Qingjuan GONG
;
Ying LIANG
;
Zhenhe LU
;
Jinsheng CHEN
;
Qiaodong HUANG
- Publication Type:Journal Article
- Keywords:
Src-family kinases;
Neuropathic pain;
Microglia;
Spinal dorsal horn
- From:
The Journal of Practical Medicine
2016;32(16):2594-2598
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the roles of spinal microglial Src-family kinases (SFKs) in diabetic neuropathic pain. Methods Male Sprague-Dawley rats, weighing 200 ~ 220 g, were used in the experiments. The level of p-SFKs in spinal dorsal horn was detected by single immunofluorescence staining on day 7th , 14th and 28th after intraperitoneally injection of Streptozotocin (STZ) and its location was detected by double immunofluorescence staining. The changes of 50% paw-withdrawal thresholds of rat were detected by behavioral tests when PP2 , a specific inhibitor of SFKs , was intrathecally administered before intraperitoneally injection of STZ. Results Compared with vehicle group, the blood glucose level increased on day 1 (P < 0.001) and the hyperglycemia persisted at least for 28 days (P < 0.001) after intraperitoneally administered of STZ (50 mg/kg). Paw-withdrawal threshold (PWT) decreased gradually from day 1 (P < 0.05) and reached the minimum on day 28 (P < 0.001) after STZ administration. Meanwhile, the expression of p-SFKs in spinal dorsal horn markedly increased on day 7 (P < 0.01), day 14 (P < 0.01) and day 28 (P < 0.01). The p-SFKs was mainly co-localized only with microglia , but not with neurons or with astrocytes. Intrathecally administered of PP2 before STZ reversed STZ-induced mechanical hyperalgesia. Conclusion Microglial SFKs in spinal dorsal horn maybe play a pivotal role in diabetic neuropathic pain.
