Effect of micellar polymer PEG-PLL-OPG on osteoporosis and arteriosclerosis in mice
10.3969/j.issn.1006-5725.2016.16.003
- VernacularTitle:纳米胶束复合物PEG-PLL-OPG对小鼠骨质疏松及动脉硬化的影响
- Author:
Chunbian CUI
;
Jing WANG
;
Yingchun ZHAO
;
Jiwei ZHANG
;
Yuqian ZHAO
;
Yantong YANG
- Publication Type:Journal Article
- Keywords:
Osteoporosis drugs;
Arteriosclerosis;
Osteoprotegerin;
Osteoblast;
Gene therapy
- From:
The Journal of Practical Medicine
2016;32(16):2598-2602
- CountryChina
- Language:Chinese
-
Abstract:
Objective By using PEG-PLL and OPG-expressing plasmid to synthesize a nanocompound PEG-PLL-OPG (PPO), to investigate its effects on osteoblasts (OB) and arteriosclerosis (AS) in mice. Methods The incidences of osteoporosis (OP) and AS were studied in people. Collect people′s serum , and divide them into different groups According to the results of diagnosis, the patients were grouped into the groups of Control, AS, OP, and AS. PPO was used to deal with OB in different groups. The cell apoptosis, cell activity, the adhersion and calcification to scaffold PCL were determined by flow cytometry, MTT, alizarin red stain and SEM,respectively. PPO was also injected intp the ApoE-/-RANKL+/+ mice via caudal vein to demonstrate its effects on BMD and AS. Results PPO could increase the cell activity , inhibit OB apoptosis and promote cell adhersion and calcifica-tion on scaffold PCL in vitro. PPO could also decrease the area and calcification of atheromatous plaque , and in-crease the BMD of collum femoris in vivo. Conclusion PPO may be a new drug for OP and AS treatments.
