Inhibiting effect of Lactobacillus acidophilus on intestinal colonization of enterohaemorrhagic E.coli O157:H7 in vitro and vivo
10.3969/j.issn.1006-5725.2016.16.004
- VernacularTitle:嗜酸乳杆菌抑制大肠杆菌O157：H7肠道定植体内外实验
- Author:
Ruqin LIN
;
Xianbo WU
;
Yiduo ZHANG
;
Yawen LI
;
Yuhua WU
;
Hongying FAN
- Publication Type:Journal Article
- Keywords:
E.coli O157:H7;
Lactobacillus acidophilus;
Probiotics;
Adhesion;
Colonization
- From:
The Journal of Practical Medicine
2016;32(16):2603-2607
- CountryChina
- Language:Chinese
-
Abstract:
Objective To test the inhabiting effect of Lactobacillus acidophilus on E.coli O157: H7 in intestinal colonization and explore its mechanism. Methods The suppressive effects of Lactobacillus acidophilus against E.coli O157:H7 adhering to Ht29 cells were carried out by competition , exclusion and replacement as-says. Furthermore, we evaluated the cytokine levels of IL-4, IL-12, and INF-γ in serum of mice. In addition, E.coli O157:H7 fecal shedding was monitored and the pathological changes of intestines were observed in mice. Results The competition, exclusion and replacement assays showed Lactobacillus acidophilus inhibited E.coli O157:H7 adhering to Ht29 cells. In vivo, the mice of treatment group were induced significantly higher level of IL-4, IL-12, and INF-γ, though prevention group induced IL-12 only. Fifteen days after E.coli O157:H7 infec-tion, there were 8 mice (80%) in prevention group and 5 mice (50%) in treatment group stopped shedding. Moreover, the pathological changes of intestines of both prevention group and treatment group appeared normal , but control groups showed seriously damaged in intestinal villus. Conclusion Lactobacillus acidophilus inhibits E.coli O157:H7 in intestinal colonization and the preventative effect was better than treatment effect. Thus , Lac-tobacillus acidophilus can be used for E.coli O157:H7 in prevention and treatment infection as probiotics.
