Influence of Let-7f regulation on MMP-11 in migration and invasion of pancreatic cancer
10.3969/j.issn.1006-5725.2016.16.005
- VernacularTitle:Let-7f调节MMP-11影响胰腺癌侵袭迁移
- Author:
Haohai JIANG
;
Xiangming MU
;
Xiaohong LI
;
Jingjing LU
;
Yuhua LU
;
Dechun LI
- Publication Type:Journal Article
- Keywords:
Pancreatic cancer;
Let-7f;
MMP-11;
Migration;
Invasion
- From:
The Journal of Practical Medicine
2016;32(16):2608-2612
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the influence of Let-7f regulation on MMP-11 in migration and in-vasion of pancreatic cancer. Methods RT-qPCR was performed to assay the expression of Let7 and MMP-11 ex-pression in pancreatic cancer cells and normal pancreatic cells. Let-7f mimic and inhibitor were transfected to PANC-1 and Bx-PC3, respectively. The influence of Let-7f expression on migration and invasion of pancreatic cancer cells was analyzed by transwell. The influence of Let-7f intervention on MMP-11 expression was observed by RT-qPCR. Results Let-7f expression decreased in PANC-1 and Bx-PC3, compared to HPDE6c7, while MMP-11 expression up-regulated in PANC-1 and Bx-PC3 , compared to HPDE6c7 cells. Let-7f overexpression in-hibited the expression of MMP-11. Up-regulated Let-7f could significantly inhibit migration and invasion of PANC-1 and Bx-PC3 and Let-7f inhibitor enhanced MMP-11 expression. However , down-expression of Let-7f could not significantly promote migration and invasion abilities of PANC-1 and Bx-PC3cells. Conclusions Let-7f may affect migration and invasion by regulating MMP-11 expression in pancreatic cancer , suggesting that Let-7f might be a potential target for gene therapy in human pancreatic cancer.
