An experimental study of bio-polymer composite film as a micro-skin autograft covering
10.3969/j.issn.1006-5725.2016.16.007
- VernacularTitle:高分子生物复合膜作为微粒皮移植覆盖物的实验研究
- Author:
Ying XIE
;
Chongyan LENG
;
Hong WANG
;
Wende CAO
;
Wuquan LI
;
Ying PENG
;
Yang ZHAO
- Publication Type:Journal Article
- Keywords:
Micro-skin graft;
Bio-polymer composite film;
Acelluar porcine skin
- From:
The Journal of Practical Medicine
2016;32(16):2617-2621
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the promoting effects of bio-polymer composite film as a micro-skin auto-graft covering on wound healing. Methods The full thickness skin defect models were made on both sides of 30 experimental rabbits. Then, the rabbits were randomly divided into experimental group and control group. In the for-mer group, the side was covered with chitosan/glucomannan composite membrane and in the latter, the side cov-ered with acelluar porcine skin after micro-skin autograft. We obtained wound tissues at week 1 , 2, 3, 4 and 5 af-ter operation. The conditions of wound healing were observed, the rate of wound healing was calculated, HE stain-ing was made, and PCNA and CD31 were detected by immunohistochemistry. Results (1) During 2~4 weeks af-ter operation, the rate of wound healing in the experimental group was significantly higher than that of the control wound (P<0.01). (2) The amount of neutrophil in experimental group was less than that of the control after oper-ation. (3) During 1 ~ 2 weeks after operation, the expression of PCNA in the experimental group was higher than that of the control group (P < 0.01), but lower than the control wounds during 1 ~ 2 weeks after operation (P <0.01). (4) During 1 ~ 5 weeks after operation, the expression of CD31 in the experimental group was higher than that of the control group (P < 0.01). Conclusion Chitosan/glucan-mannan composite membrane as a micro-skin autograft covering may promote wound healing.
