The effect of COH and IVM on mitochondrial membrane potentials and cytoskeleton
10.3969/j.issn.1006-5725.2016.16.009
- VernacularTitle:控制性超排卵和体外成熟培养对卵子线粒体膜电位及细胞骨架的影响
- Author:
Mimi DAI
;
Hongshan GE
;
Jieqiang LV
- Publication Type:Journal Article
- Keywords:
COH;
IVM;
mitochondrial membrane potentials;
cytoskeleton
- From:
The Journal of Practical Medicine
2016;32(16):2625-2628
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of different maturation methods on mitochondrial functions of oocytes and the possible mechanism. To explore novel ideas for developing assisted reproductive technology (ART). Methods Female mice were used as models and randomly allocated into three groups, COH, IVM and NC control. Oocytes maturated with different methods which were all simulated with those treatments in human IVF cycle. Immunofluorescence were used to measure the mitochondrial membrane potentials and analyze the cy-toskeleton. Results The mitochondrial membrane potential in the COH group was significantly lower than that in NC group and IVM group (P < 0.05). The proportion of normal cytoskeleton including spindle structure and chromosome configuration in the COH group and IVM group were significantly lower than that in the NC group (PCOH < 0.01, PIVM < 0.05). Conclusions Both COH and IVM can affect mitochondrial functions.
